Much like in the games, most of the Nurse Joys have brink pink hair and fair skin. Others could have a lighter shade of hair color, or brighter or darker skin. In Unova, Nurse Joys have different hairstyles with the hair being tied in rounder rings, a plain pink dress with a shorter apron, white tights, and pink shoes instead of white. Kalos Nurse Joys wear a pink and white dress with a white collar, and the same tights and shoes as the ones in Unova. Alola Nurse Joys wear a pink dress with a built-on apron pocket and white flat sandals.

As revealed in Drifloon in the Wind! Surge's Raichu. She stopped Ash and Misty from fighting, reminding them that the hospital required peace. Nurse Joy then advised Ash that Pikachu would require more rest to regain its stamina for its upcoming rematch.

Pikachu inspects the room where she was and suddenly falls asleep. Ash follows it and meets the same fate. Outside, Team Rocket is knocked out of their tree by the gust from a Shiftry who is carrying away Nurse Joy. They decide they should capture it for their boss. They see the Shiftry taking Nurse Joy and decide to track it.

He says that Ash is acting like he won the whole competition after winning "two easy matches". He then says, "He better enjoy all his celebratin' now, 'cause when his luck runs out, Ash Ketchum won't have anything to cheer about. Unless he wants to cheer for me, right girls? While he is speaking, he seems jealous and a bit spiteful toward Ash. Brock's love meter skyrockets at this rate.